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Relative expressions and concentrations of IL-1β, IL-6, TNF-α, and Lp-PLA2. ( A , B , and C ) The mRNA expression levels of IL-1β, IL-6, and TNF-α in RAW264.7 cells 24 h after LPS (1 µg/mL) stimulation as measured by qRT‒PCR. ( D ) The concentrations of Lp-PLA2 as measured by ELISA. Parametric data were analysed by paired t tests and are displayed as the mean ± SD. N = 3, ** p < 0.01, **** p < 0.0001

Journal: European Journal of Trauma and Emergency Surgery

Article Title: Lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells is inhibited by microRNA-494-3p via targeting lipoprotein-associated phospholipase A2

doi: 10.1007/s00068-024-02588-7

Figure Lengend Snippet: Relative expressions and concentrations of IL-1β, IL-6, TNF-α, and Lp-PLA2. ( A , B , and C ) The mRNA expression levels of IL-1β, IL-6, and TNF-α in RAW264.7 cells 24 h after LPS (1 µg/mL) stimulation as measured by qRT‒PCR. ( D ) The concentrations of Lp-PLA2 as measured by ELISA. Parametric data were analysed by paired t tests and are displayed as the mean ± SD. N = 3, ** p < 0.01, **** p < 0.0001

Article Snippet: ELISA kits for the detection of Lp-PLA2 (CSB-E08321m, Cusabio, USA), TNF-α (SEKM-0034, Solarbio, China), IL-6 (SEKM-0007, Solarbio, China) and IL-1β (SEKM-0002, Solarbio, China) were obtained from Solarbio (Solarbio, Beijing, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Cell proliferation and luciferase assay results. ( A ) The concentrations of Lp-PLA2 as determined by ELISA. ( B ) The results of the luciferase assay showed the relative activity of Lp-PLA2, indicating that miR-494-3p mimic transfection significantly inhibited wild-type luciferase activity in the 3′-UTR of Lp-PLA2 but had no effect on the mutated 3′-UTR of Lp-PLA2 ( p < 0.05). Parametric data were analysed by paired t tests and are displayed as the mean ± SD. N = 3, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: European Journal of Trauma and Emergency Surgery

Article Title: Lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells is inhibited by microRNA-494-3p via targeting lipoprotein-associated phospholipase A2

doi: 10.1007/s00068-024-02588-7

Figure Lengend Snippet: Cell proliferation and luciferase assay results. ( A ) The concentrations of Lp-PLA2 as determined by ELISA. ( B ) The results of the luciferase assay showed the relative activity of Lp-PLA2, indicating that miR-494-3p mimic transfection significantly inhibited wild-type luciferase activity in the 3′-UTR of Lp-PLA2 but had no effect on the mutated 3′-UTR of Lp-PLA2 ( p < 0.05). Parametric data were analysed by paired t tests and are displayed as the mean ± SD. N = 3, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: ELISA kits for the detection of Lp-PLA2 (CSB-E08321m, Cusabio, USA), TNF-α (SEKM-0034, Solarbio, China), IL-6 (SEKM-0007, Solarbio, China) and IL-1β (SEKM-0002, Solarbio, China) were obtained from Solarbio (Solarbio, Beijing, China).

Techniques: Luciferase, Enzyme-linked Immunosorbent Assay, Activity Assay, Transfection

Fig. 1 Relative expressions and concentrations of IL-1β, IL-6, TNF-α, and Lp-PLA2. (A, B, and C) The mRNA expression levels of IL-1β, IL-6, and TNF-α in RAW264.7 cells 24 h after LPS (1 µg/mL) stimulation as measured by qRT‒PCR. (D) The concentrations of Lp-PLA2 as measured by ELISA. Parametric data were analysed by paired t tests and are displayed as the mean ± SD. N = 3, ** p < 0.01, **** p < 0.0001

Journal: European journal of trauma and emergency surgery : official publication of the European Trauma Society

Article Title: Lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells is inhibited by microRNA-494-3p via targeting lipoprotein-associated phospholipase A2.

doi: 10.1007/s00068-024-02588-7

Figure Lengend Snippet: Fig. 1 Relative expressions and concentrations of IL-1β, IL-6, TNF-α, and Lp-PLA2. (A, B, and C) The mRNA expression levels of IL-1β, IL-6, and TNF-α in RAW264.7 cells 24 h after LPS (1 µg/mL) stimulation as measured by qRT‒PCR. (D) The concentrations of Lp-PLA2 as measured by ELISA. Parametric data were analysed by paired t tests and are displayed as the mean ± SD. N = 3, ** p < 0.01, **** p < 0.0001

Article Snippet: ELISA kits for the detection of Lp-PLA2 (CSB-E08321m, Cusabio, USA), TNF-α (SEKM-0034, Solarbio, China), IL-6 (SEKM-0007, Solarbio, China) and IL-1β (SEKM0002, Solarbio, China) were obtained from Solarbio (Solarbio, Beijing, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Fig. 2 Cell proliferation and luciferase assay results. (A) The concen trations of Lp-PLA2 as determined by ELISA. (B) The results of the luciferase assay showed the relative activity of Lp-PLA2, indicating that miR-494-3p mimic transfection significantly inhibited wild-type luciferase activity in the 3′-UTR of Lp-PLA2 but had no effect on the mutated 3′-UTR of Lp-PLA2 (p < 0.05). Parametric data were anal ysed by paired t tests and are displayed as the mean ± SD. N = 3, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: European journal of trauma and emergency surgery : official publication of the European Trauma Society

Article Title: Lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells is inhibited by microRNA-494-3p via targeting lipoprotein-associated phospholipase A2.

doi: 10.1007/s00068-024-02588-7

Figure Lengend Snippet: Fig. 2 Cell proliferation and luciferase assay results. (A) The concen trations of Lp-PLA2 as determined by ELISA. (B) The results of the luciferase assay showed the relative activity of Lp-PLA2, indicating that miR-494-3p mimic transfection significantly inhibited wild-type luciferase activity in the 3′-UTR of Lp-PLA2 but had no effect on the mutated 3′-UTR of Lp-PLA2 (p < 0.05). Parametric data were anal ysed by paired t tests and are displayed as the mean ± SD. N = 3, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: ELISA kits for the detection of Lp-PLA2 (CSB-E08321m, Cusabio, USA), TNF-α (SEKM-0034, Solarbio, China), IL-6 (SEKM-0007, Solarbio, China) and IL-1β (SEKM0002, Solarbio, China) were obtained from Solarbio (Solarbio, Beijing, China).

Techniques: Luciferase, Enzyme-linked Immunosorbent Assay, Activity Assay, Transfection